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KMID : 0613820020120030369
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Journal of Life Science
2002 Volume.12 No. 3 p.369 ~ p.373
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Production of Microbial Pesticides by Soybean Curd Waste-water in Bacillus thuringiensis subsp. kurstaki HD-1
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Ok Min
Kim Dae-Jin
Lee Young-Chun
Choi Yong-Lark
Cho Young-Su
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Abstract
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The waste-water from the industry for production of a soybean curd (the soybean curd waste-water) was investigated to use for the substrate to produce the endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 used as one of well known microbial pesticides. The pH of the soybean curd waste-water was 9.8 and its chemical oxygen demand (COD), total nitrogen (TN) and phosphate (TP) were 276.0, 71.1 and 5.5㎎/ℓ, respectivery. The higher was the concentration of the soybean curd waste-water in the medium, the more endotoxin was produced. Maximal sporulation occurred at which concentration of K2HPO4 in the medium supplied with the soybean curd waste-water was 1% (w/v). Production of the endotoxin with the optimized medium supplied with the soybean curd waste-water was 1.5 times higher than that without the soybean curd waste-water. The soybean curd waste-water was found to be suitable substrate for production of the endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1.
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KEYWORD
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endotoxin, microbial pesticide, soybean curd waste-water, Bacillus thuringiensis kurstaki HD-1
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